c57bl 6j wild type females  (Jackson Laboratory)

Journal: Frontiers in Immunology

Article Title: Nerve growth factor responsive elements modulate immune cell inflammation and are dysregulated in an Alzheimer’s disease mouse model

doi: 10.3389/fimmu.2026.1722477

Figure Lengend Snippet: Changes in NGF receptors expression upon splenocyte activation: Spleen dissected from wild type C57BL/6J mice were processed to isolate splenocytes and plated as 1 million cells per well in the 96-well plate. After 48hr stimulation either with PMA and iono (PMA:100 ng/ml; iono: 2 μg/ml) or LPS (1 μg/ml), splenocytes were examined for TrkA and p75 by flow cytometry. (A) Histograms present expression of TrkA and p75 in unstimulated (black line), PMA-iono treated (red line) or LPS treated (blue line) T (CD3 + gated) and B (B220 + gated) cells. Unstained controls were displayed as grey dotted line. (B) Bar plot presents percent change in mean fluorescence intensity (MFI) of TrkA and p75 in stimulated groups (PMA-iono or LPS) compared to respective unstimulated (US) group in T (CD3 + gated) and B (B220 + gated) cells. Data are represented as mean ± S.D. and the statistical comparison across different groups was analysed by two-way ANOVA with Tukey’s multiple comparisons test (three independent experiments; n=3 mice); *p < 0.05, ***p < 0.001, ****p < 0.0001.

Article Snippet: In this study, female wild-type (WT) C57BL/6J (from Janvier labs, France) and App NL-G-F knock-in (NLGF) mice were used.

Techniques: Expressing, Activation Assay, Flow Cytometry, Fluorescence, Comparison

Journal: Frontiers in Immunology

Article Title: Nerve growth factor responsive elements modulate immune cell inflammation and are dysregulated in an Alzheimer’s disease mouse model

doi: 10.3389/fimmu.2026.1722477

Figure Lengend Snippet: Effect of NGF treatment on cytokine production in splenocytes. Spleens dissected from wild type C57BL/6J mice were processed to isolate splenocytes and plated in 96 well plate with 1 million cells per well. Splenocytes were treated with either PMA-iono (PMA:100 ng/ml; iono: 2 μg/ml) or PMA-iono supplemented with 100 ng/ml mature-NGF for 48hr and examined for TNF-α cytokine expression in CD3 + T cells and B220 + B cells by flow cytometry. The bar diagram presents percent change in mean fluorescence intensity (MFI) of TNF-α in stimulated groups (PMA-iono or PMA-Iono + NGF) compared to respective unstimulated (US) group in T (CD3 + ) and B (B220 + ) cells. Data are represented as mean ± S.D. and statistical comparison across different groups (performed as three independent experiments, n=3 mice) were analysed by one-way ANOVA with Tukey’s correction for multiple comparisons; *p < 0.05, **p < 0.01, ***p < 0.001.

Article Snippet: In this study, female wild-type (WT) C57BL/6J (from Janvier labs, France) and App NL-G-F knock-in (NLGF) mice were used.

Techniques: Expressing, Flow Cytometry, Fluorescence, Comparison